Quiescence is a ubiquitous cell cycle stage conserved from microbes through humans and is essential to normal cellular function and response to changing environmental conditions.We recently reported a massive repressive event associated with quiescence in Saccharomyces cerevisiae, where Cooktop Bench Seal Rpd3 establishes repressive chromatin structure that drives transcriptional shutoff [6].Here, we describe in detail the experimental procedures, data collection, and data analysis related to our characterization of transcriptional Stevia quiescence in budding yeast (GEO: GSE67151).Our results provide a bona fide molecular event driven by widespread changes in chromatin structure through action of Rpd3 that distinguishes quiescence as a unique cell cycle stage in S.
cerevisiae.